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Article in Japanese
Clinical evaluation of PCR assays for detection of periodontal bacteria in patients with lung abscesses or empyemata
Kotaro Kadoyaa Yasuko Yoshiokaa Yukiko Nambaa Yoko Katsuraa Ryo Koa Kengo Koikea Masakata Yoshiokaa Shinichi Sasakia Shigeru Tominagaa Kazuhisa Takahashib
aDepartment of Respiratory Medicine, Juntendo University Urayasu Hospital
bDepartment of Respiratory Medicine, Juntendo University School of Medicine
Current evidence suggests that periodontal disease may be associated with the development of a lung abscess or an empyema. Some microbes, including the Streptococcus anginosus group and obligate anaerobic bacteria, are recognized as important pathogens. We analyzed periodontal disease bacteria by polymerase chain reaction (PCR) assays of transbronchial biopsy (TBB) specimens, bronchial lavage fluid (BLF), or pleural effusion obtained from 7 cases (lung abscess, 3; and empyema, 4). In 1 case, anaerobes were isolated from BLF culture, but periodontal pathogens were detected in TBB specimens or pleural effusion by PCR assays in 6 cases (Porphyromonas gingivalis, 3; Tannerella forsythensis, 1; Treponema denticola, 1; and Prevotella intermedia, 1). In 1 case T. denticola of unknown pathogenicity was detected in the TBB specimens. PCR assays targeting periodontal disease bacteria are potentially useful for identifying the causes of respiratory infectious diseases, which are difficult to diagnose by anaerobic culture.
Lung abscess Empyema Periodontal bacteria PCR assay Treponema denticola
Received 30 Sep 2011 / Accepted 17 Jul 2012
AJRS, 2(2): 79-84, 2013