 |
Vol.39 No.2 contents | Japanese/English |
- Original Article -
Atypical Adenomatous Hyperplasia and Well Differentiated Adenocarcinoma: Analysis by Morphometry, DNA index and Immunohistochemistry
Chimaengul Wushur1,2, Yoshiro Ebihara2, Harubumi Kato3, Yuichi Ichinose1 and Keisuke Toyama1First Department of Internal Medicine1, Second Department of Pathology2 and First Department of Surgery3, Tokyo Medical University
In order to know whether atypical adenomatous hyperplasia (AAH) is a precursor or early stage of well differentiated adenocarcinoma (WDA), we compared the histology of AAH and well differentiated adenocarcinomas morphologically. Materials consisted of 135 lesions of either AAH and WDA collected from 41 resected lung specimens at Tokyo Medical University Hospital between 1995 and 1997. AAH lesions were classified into low-grade AAH (AAH-L), moderate-grade AAH (AAH-M), and high-grade AAH (AAH-H) histologically, and the nuclear DNA ploidy patterns, and the expressions of p53, Ki-67 and cyclin D1 were analyzed with an image cytometer and immunohistochemistry using monoclonal antibodies. Nuclear-DNA ploidy analyses revealed that 81.8% of WDAs were aneuploid, as were 66.7%, 31% and 24.3%cases of high, moderate and low AAHs respectively. Expression of p53, Ki-67, and cyclin D1 increased as the grade of AAHs increased, and was highest of all in carcinomas. All four parameters showed a tendency towards more aggressiveness in the low and moderate degrees of carcinoma-associated AAH than those of isolated AAH. These results suggest that AAHs could be a precursor lesion of WDA. In addition, there seem to be differences at the genetic level between isolated AAHs and carcinoma-associated AAHs even if they have the same degree of morphological abnormality.
key words: Atypical adenomatous hyperplasia, DNA ploidy pattern, Immunohistochemistry, Type II pneumocyte, Clara cell
Received: November 10, 1998
Accepted: February 12, 1999
JJLC 39 (2): 125-135, 1999
