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Vol.47 No.7 contents | Japanese/English |
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- The 21st Lung Cancer Workshop -
Problems in Histogenesis of Pulmonary Squamous Cell Carcinoma
Yukio Takeshima1, Kouki Inai1, Osamu Furonaka2, Takashi Nishisaka31Department of Pathology, 2Department of Molecular Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Japan, 3Department of Pathology and Laboratory Medicine, Hiroshima Prefectural Hospital, Japan
Objective. To elucidate the histogenesis of pulmonary squamous cell carcinoma in terms of the location (central or peripheral) by analyzing the various genetic alterations and protein expressions. Methods. As a model of multistep carcinogenesis of central type squamous cell carcinoma, the various preinvasive lesions including hyperplasia, dysplasia and squamous cell carcinoma in situ were collected, and analyzed the abnormalities of tumor suppressor genes, especially p53 gene. Moreover, concerning the peripheral type squamous cell carcinoma, the abnormal methylation of p14, p15 and p16 genes and p16 expression were analyzed, and moreover, the expression of differentiation markers including Maspin, p63 and TTF-1 were also immunohistochemically analyzed. Results. The accumulation of genetic alterations was observed according to the atypia in dysplasias and squamous cell carcinoma in situ. The higher frequency of abnormal methylation of various genes in central type squamous cell carcinomas was observed comparing with peripheral type. In the peripheral type squamous cell carcinomas, the methylation of p16 gene and smoking index were well correlated. Some peripheral type squamous cell carcinomas showed positivity for TTF-1, usually not detected in squamous cell carcinomas. Conclusion. The analyses of profiles of genetic and epigenetic alterations and the expressions of various genes among dysplasia and squamous cell carcinomas would be useful to speculate on the histogenesis.
key words: Lung cancer, Squamous cell carcinoma, Central type, Peripheral type, Histogenesis
JJLC 47 (7): 927-933, 2007
