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Vol.50 No.2 contents | Japanese/English |
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- Original Article -
Proteins Secreted by Lung Adenocarcinoma Cells -Identification of Candidate Diagnosis Markers from Culture Supernatants of Dissected Tumor Tissue-
Ran Guo1, Takashi Hirano2, Takao Kawakami1,3, Yunbo Gong1, Masaharu Nomura1, Gaku Yamaguchi1, Hisashi Saji1, Masatoshi Kakihana1, Tatsuo Ohira1, Norihiko Ikeda11Department of Surgery, Tokyo Medical University, Japan, 2Toda Chuo General Hospital, Japan, 3Research and Development Division, Medical ProteoScope Company, Japan
Objective. There is a need to establish clinically useful serum biomarkers for effective lung cancer screening. We investigated extracellular proteins secreted or shed from surgically resected lung adenocarcinoma tissue into its primary culture medium. Methods. In 12 lung adenocarcinoma cases, protein specimens from both culture supernatants of adenocarcinoma tissue and the normal peripheral lung (NPL) tissue were labeled with different fluorescent dyes (CyDyeTM: Cy2, Cy3 and Cy5) and co-resolved in single two-dimensional (2-D) gels. Quantitative protein profiles were obtained by differential fluorescence imaging of the gels. Results. The twelve pairs of 2-D gel images of both adenocarcinoma and NPL specimens revealed 34 distinct protein spots, detected in at least 5 gel images and highly concentrated in the adenocarcinoma cultures, on average more than double the concentration of analysis identified all of these protein spots, including napsin A and pulmonary surfactant-associated protein A which are responsible for the development of type II pneumocytes, and are the proteins commonly upregulated in tumor cells. Conclusion. These results suggest the usefulness of the tissue culture method to identify potential serum biomarkers to enable the early detection of lung adenocarcinoma.
key words: Serum biomarker, Secreted protein, Early detection, Two-dimensional difference gel electrophoresis, Lung adenocarcinoma
Received: December 2, 2009
Accepted: March 2, 2010
JJLC 50 (2): 141-150, 2010
