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Vol.59 No.2 contents | Japanese/English |
 | Full Text of PDF (324K) Article in Japanese |
- Invited Review Article -
Handling of Appropriate Histological Samples Required to Make an Accurate Lung Cancer Diagnosis
Koji Tsuta11Department of Pathology and Laboratory Medicine, Kansai Medical University, Japan
With the progression of molecular diagnostic methods, histological samples have come to be used as a source for the histological diagnosis as well as for detected protein expression and genomic alterations, which are treatment prediction factors. A formalin-fixed paraffin-embedded sample allows for easy handling in daily practice and long-term storage in most pathology departments. However, fixation with chemical modification using formalin is a well-known mechanism that affects the protein and genetic quality. We must also examine a number of factors (such as cold ischemic time, composition of formalin, quantity of the fixative, fixation time, and paraffin temperature) and determine the appropriate conditions in order to minimize the negative effects on constructing the material.
key words: Formalin fixed paraffin-embedded sample, Cold ischemic time, Composition of formalin, Fixation time, Paraffin temperature
JJLC 59 (2): 123-127, 2019
