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Vol.64 No.1 contents | Japanese/English |
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- Original Article -
An Exploratory Study of Specimen Processing Methods for Successful Multiplex Genetic Analyses Using Lung Surgical Specimens
Yosuke Matsuura1, Ken Uchibori2, Hironori Ninomiya3, Junji Ichinose1, Masayuki Nakao1, Sakae Okumura1, Makoto Nishio2, Mingyon Mun11Department of Thoracic Surgical Oncology, 2Department of Thoracic Medical Oncology, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Japan, 3Division of Pathology, Cancer Institute, Japanese Foundation for Cancer Research, Japan
Objective. Perioperative treatment of non-small cell lung cancer is becoming more precise, and multiplex genetic analyses using next generation sequencing will inevitably be required even for surgical cases. Appropriate specimen processing is important for successful multiplex genetic analyses of surgical specimens. We therefore validated the specimen processing method for lung surgery at our hospital and obtained indicators that should be observed for successful multiplex genetic analyses from surgical specimens. Methods. Thirty patients with clinical stage IA3-IIIA non-small cell lung cancer scheduled for surgery were prospectively enrolled. Rapid intraoperative specimens were fixed in 10% formalin and surgical specimens in 20% formalin, and each was submitted to the Oncomine™ Dx Target TestⓇ Multi-CDx System (ODxTT). The success rate of the ODxTT analysis of each specimen and the quantity of nucleic acids extracted from each specimen were compared. The correlation between the quantity of nucleic acids extracted from the surgical specimens and the time required for the specimen processing process was also evaluated. Results. The success rate of the ODxTT analysis was 100% for both specimens. The extracted quantities of nucleic acids of both DNA and RNA were significantly higher in the surgical specimens than the rapid intraoperative specimens. No significant correlation was observed between the quantity of nucleic acids and the time required for specimen processing. Conclusion. The success rate of the ODxTT analysis was 100% for lung surgical specimens fixed in 20% formalin. The use of 20% formalin may be considered in institutions where it is difficult to process surgical specimens as recommended.
key words: Non-small cell lung cancer, Next generation sequencing, Oncomine™ Dx Target TestⓇ, Surgical specimens, Quantity of nucleic acid
Received: September 26, 2023
Accepted: October 17, 2023
JJLC 64 (1): 11-16, 2024
