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第41巻第2号目次 | Japanese/English |
 | Full Text of PDF (108K) Article in Japanese |
─ 原著 ─
Integrins α2β1, α5β1 and αvβ5 Are Related to Tumor Growth and Metastasis of Non-small Cell Lung Cancer
Tatsuya Yoshimasu1), Nariaki Matsuura2), Ichiro Ota2), Naoyuki Tani2), Shoji Oura1), Hirokazu Tanino1), Teruhisa Sakurai1), Yozo Kokawa1), Kenji Matsuyama1) and Yasuaki Naito1)1)Department of Thoracic and Cardiovascular Surgery, Wakayama Medical University, Wakayama 641-0012, Japan, 2)Department of Pathology, School of Allied Health Sciences, Faculty of Medicine, Osaka University, Osaka 565, Japan
Objective: Integrins are related to the growth, invasion, and metastasis of cancer cells, and lung cancer cells express multiple integrin subunits. This study was designed to determine the integrin subunits which play major roles in the growth and metastasis of non-small cell lung cancer (NSCLC). Methods: NSCLC cell lines EBC-1, LK-2, LC-1F, PC-14, PC-3, VMRC-LCD and IA-5 were transplanted into athymic mice both subcutaneously and intravenously. Subcutaneous tumor sizes were measured twice a week. Carcinoma cells from evident metastatic lesions were obtained and cultured. The patterns of integrin subunit expression were evaluated using immunofluorescence flowcytometric analysis. Results: Maximal subcutaneous tumor size in the exponential growth phase was negatively correlated with integrin α5 and β1 expression (p=0.028, p=0.035). Three months after transplantation, carcinoma cells from 11 evident metastatic lesions were obtained and cultured. Expression of integrins α1, α2, α3,α4, α5, α6, αv, β1, β3, β4 and β5 was evaluated by flowcytometry. Expression of integrins α2,α3,α5,α6,β1,β4 and β5 was commonly detected in original cell lines. The evident changes at the sites of metastasis were increases in α2 (p=0.04), αv (p=0.05), β1 (p=0.008) and β5 (p=0.09) expression. Conclusion: These results suggest that integrins α2β1 and αvβ5 are related to metastasis, and that α 5β 1 is related to tumor growth of NSCLC.
索引用語：Flowcytometry, Integrin, Metastasis, Non-small cell lung cancer
受付日：2000年8月17日
受理日：2001年2月8日
肺癌 41 (2)：111─115，2001
