The Journal of the Japanese Respiratory Society ONLINE JOURNAL
ABSTRACT
Full Text of PDF (44k)
Article in Japanese
Original Article
Genetic Variation of NADPH/NADH Oxidase and Susceptibility to Diffuse Panbronchiolitis (DPB) and Chronic Obstructive Pulmonary Disease (COPD)
Takeo Ishii1) , Naoto Keicho2) , Shinji Teramoto3) , Arata Azuma4) , Shoji Kudoh4) , Yoshinosuke Fukuchi5) , Yasuyoshi Ouchi1) and Takeshi Matsuse6)
1)Department of Geriatric Medicine, University of Tokyo, Tokyo 113-8655, 2)Department of Respiratory Diseases, Research Institute, International Medical Center of Japan, Tokyo 162-8655, 3)Department of Internal Medicine, San-no Hospital, Medical Research Center, International University of Health and Welfare, Tokyo 107-0052, 4)Fourth Department of Internal Medicine, Nippon Medical School, 5)Department of Respiratory Medicine, Juntendo University of School of Medicine, Tokyo 113-8421, Japan, 6)Department of Pulmonary Medicine, Yokohama City University Medical Center, Yokohama 232-0024
ABSTRACT
Diffuse panbronchiolitis (DPB) and chronic obstructive pulmonary disease (COPD) are both characterized by chronic airflow obstruction of unknown etiology. It is hypothesized that neutrophils play the major role in the pathogenesis of these diseases. Recent studies have suggested that genetic factors may be related to individual susceptibility to these diseases. We have investigated the association between the C 242 T polymorphism of p 22 phox, a critical subunit of superoxide-generating NADH/NADPH oxidase, and susceptibility to DPB and COPD. Blood samples obtained from both patients with DPB (n=82), COPD (n=53), and control subjects (n=82) were used for this genotyping assay; and the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were performed to genotype the gene. The frequency of the C allele was 0.91, 0.92, 0.92 in the DPB, COPD, and control groups, respectively. There were no differences in the distribution of the genotype of p 22 phox among these groups, either. We concluded that there is no association between the C 242 T polymorphism of p 22 phox, and susceptibility to DPB and COPD.
KEYWORDS: NADPH/NADH oxidase, Polymorphism, Diffuse panbronchiolitis (DPB), Chronic obstructive pulmonary disease (COPD)
RECEIVED: 2000.9.27
JJRS, 39(5): 328-332, 2001
