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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Accuracy of Enterobacter cloacae complex species identified by Vitek MS |
| Language |
J |
| AuthorList |
Rino Matsui1), Takuya Hosoda1), Mitsutaka Wakuda1), Yohei Doi2,3), Masahiro Suzuki2) |
| Affiliation |
1) Department of Joint Research Laboratory of Clinical Medicine, Fujita Health University Hospital
2) Department of Microbilogy, Fujita Health University School of Medicine
3) Department of Infectious Diseases, Fujita Health University School of Medicine |
| Publication |
J.J.C.M.: 31 (2), 113-115, 2021 |
| Received |
July 14, 2020 |
| Accepted |
December 24, 2020 |
| Abstract |
Enterobacter cloacae complex consists of at least 22 species, but they are difficult to distinguish based on biochemical identification. Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has improved ability to distinguish bacterial species, and MALDI-TOF MS instruments are starting to report E. cloacae complex at species level in clinical laboratories. The aim of this work was to compare species identification results of E. cloacae complex clinical isolates using VITEK MS and whole genome sequencing based on average nucleotide identity. To this end, a total of 54 unique E. cloacae complex bloodstream isolates collected between 2014 and 2018 were studied. Complete agreement and partial agreement were seen in 1 isolate and 8 isolates between the two methods respectively, representing 17% of the study isolates. The results suggested that E. cloacae complex species should be reported as such even when species were identified by VITEK MS. |
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