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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.31 No.3 contents] Japanese / English |
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| ArticleTitle | Multicenter evaluation of clinical performance of C. DIFF QUIK CHEK COMPLETE for the detection of Clostridioides difficile and its toxin with colonies from stool cultures |
| Language | J |
| AuthorList | Toshinori Hara1,2), Hiromichi Suzuki3), Tadatomo Oyanagi4), Norito Koyanagi5), Akihito Ushiki6), Naoki Kawabata7), Miki Goto8), Yukio Hida9), Yuji Yaguchi10), Kiyoko Tamai10), Shigeyuki Notake11), Keiichi Uemura5), Seiya Kashiyama1,2), Toru Nanmoku8), Satoshi Suzuki12), Hiroshi Yamazaki7), Hideki Kimura9), Hiroyuki Kunishima13), Hiroki Ohge14) |
| Affiliation |
1) Section of Clinical Laboratory, Department of Clinical Practice and Support, Hiroshima University Hospital 2) Division of Clinical Laboratory Medicine, Hiroshima University Hospital 3) Division of Infectious Diseases, Department of Medicine, Tsukuba Medical Center Hospital 4) Department of Clinical Laboratory, St. Marianna University School of Medicine Hospital 5) Department of Clinical Laboratory, Chutoen General Medical Center 6) Department of Clinical Laboratory, Tone Chuo Hospital 7) Department of Clinical Laboratory, Tsuruga Municipal Hospital 8) Department of Clinical Laboratory, University of Tsukuba Hospital 9) Department of Clinical Laboratory, University of Fukui Hospital 10) Miroku Medical Laboratory Inc. 11) Department of Clinical Laboratory, Tsukuba Medical Center Hospital 12) Division of General Medicine, Tone Chuo Hospital 13) Department of Infectious Diseases, St. Marianna University School of Medicine 14) Department of Infectious Diseases, Hiroshima University Hospital |
| Publication | J.J.C.M.: 31 (3), 186-191, 2021 |
| Received | November 2, 2020 |
| Accepted | March 2, 2021 |
| Abstract | We prospectively analyzed the clinical performance of C. DIFF QUIK CHEK COMPLETE for detection of Clostridioides difficile and its toxin using colonies from anaerobic stool cultures isolated on C. difficile agar (CCMA-EX). Mass spectrometry and real-time PCR were used to validate the detection of Clostridioides difficile and its toxin genes. Sample collection was performed at 8 institutions between November 2018 and March 2019, and 85 strains of Clostridioides difficile were isolated from 85 stool samples. The turbidity of each isolated colony suspensions was adjusted to 3 McFarland standards before testing. Detection of glutamate dehydrogenase using C. DIFF QUIK CHEK COMPLETE showed positive results for all 85 C. difficile colonies, and toxin analysis of C. DIFF QUIK CHEK COMPLETE showed positive results for 60 C. difficile colonies. The concordance rate for toxin detection was 100% (85/85) for overall concordance, 100% (60/60) for positive concordance, and 100% (25/25) for negative concordance. In this study, we confirmed that C. DIFF QUIK CHEK COMPLETE can be used for the detection of Clostridioides difficile and its toxin with colonies from stool cultures. |
| Keywords | Clostridioides difficile, Clostridioides difficile infection, toxigenic culture |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |