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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of MALDI-TOF MS and phylogenetic analysis for clinical isolates of morphologically identified Aspergillus niger |
| Language |
J |
| AuthorList |
Yasunobu Matsumoto, Makoto Suzuki, Hiroyoshi Nihei, Satoru Matsumoto, Takashi Mikawa |
| Affiliation |
LSI Medience Corporation. Microbiological Testing Group, Infectious Diseases Testing Department |
| Publication |
J.J.C.M.: 32 (1), 23-28, 2022 |
| Received |
January 26, 2021 |
| Accepted |
August 4, 2021 |
| Abstract |
Thirty-six strains of filamentous fungi isolated in various specimens from medical facilities in Japan morphologically identified as Aspergillus niger from January, 2016 to February, 2019 were used. The purpose of this study was the evaluation of species identification of these strains by both phylogenetic analysis and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Isolates were identified as Aspergillus tubingensis, Aspergillus welwitschiae and Aspergillus neoniger in 47.2%, 50.0% and 2.8%, respectively, and there was no A. niger by phylogenetic analysis. On the other hand, identification results by MALDI-TOF MS were to the extent of A. niger or A. niger complex. Therefore, identification of Aspergillus section Nigri (A. sect. Nigri) must be performed by phylogenetic analysis at present. These results indicate improvement of MALDI-TOF MS database with convenient and prompt method due to urgent identification of resistant and low drug susceptibility species/strain in A. sect. Nigri that were recently reported. |
| Keywords |
Aspergillus niger, Aspergillus section Nigri, MALDI-TOF MS |
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