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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Prevalence and molecular epidemiological characterization of Panton-Valentin luekocidin-carrying methicillin-resistant Staphylococcus aureus strains isolated from diverse specimen sources at a university hospital, Japan |
| Language |
J |
| AuthorList |
Hirofumi Toda1), Shuichi Kubo1), Yuji Tanaka2), Toshinori Kamisako1,2), Koichiro Yoshida3) |
| Affiliation |
1) Department of Clinical Laboratory, Kindai University Hospital
2) Department of Clinical Laboratory Medicine, Kindai University Faculty of Medicine
3) Department of Medical Safety Administration, Division of Infection Control and Prevention, Kindai University Hospital |
| Publication |
J.J.C.M.: 32 (1), 33-37, 2022 |
| Received |
July 18, 2021 |
| Accepted |
September 14, 2021 |
| Abstract |
We investigated the prevalence of Panton-Valentin leucocidin (PVL) genes among methicillin-resistant Staphylococcus aureus (MRSA) isolates originating from a variety of specimen sources and determined molecular epidemiologic characteristics of PVL-positive MRSA strains. From 2016 through 2018, a total of 947 strains of MRSA isolated from 569 respiratory samples, 189 skin and soft tissue samples, 38 urinary and genital organ samples, 29 blood samples, and 122 other samples, were collected at Kindai University Hospital. All MRSA isolates were screened by polymerase chain reaction (PCR) for the presence of PVL genes. Isolates that carried the PVL genes were subsequently tested for the presence of the arginine catabolic mobile element (ACME) genes and were further characterized via Staphylococal cassette chromosome mec (SCCmec) typing, multilocus sequence typing (MLST), and PCR based open-reading frame typing (POT). 12 of 947 (1.3%) clinical isolates obtained from 2016 to 2018 harbored the PVL genes. Prevalence of PVL-positive MRSA has increased over time from 0.3% in 2016, to 0.9% in 2017 and to 2.8% in 2018. The majority of PVL-positive strains represented ST8 harboring SCCmec type IV, also known as USA300. They all were positive for ACME genes and exhibited the same POT type (POT scores, 106-77-113). In summary, the present study revealed a gradually increasing prevalence of PVL-positive MRSA, and also clarified that ACME-positive ST8-SCCmec type IV strain is the most prevalent clone in MRSA isolates collected at our hospital. Nationwide surveillance is needed to assess the trends in the molecular epidemiology of MRSA to control PVL-positive MRSA causing diverse infections. |
| Keywords |
Methicillin-resistant Staphylococcus aureus, Panton-Valentine leukocidin |
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