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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.32 No.1 contents] Japanese / English |
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| ArticleTitle | Usefulness of IR biotyper in strain level identification of Citrobacter freundii complex with reversible Shigella spp.-like properties |
| Language | J |
| AuthorList | Shun Taguchi1), Kenta Yamaguchi1), Mayo Katsuki1), Yukari Sano1), Takayuki Hirano1), Michio Yasunami1), Mami Fukuoka2), Nohara Tsukamoto3,4), Yuya Hirata5), Azumi Fujinaga6), Zenzo Nagasawa4,7) |
| Affiliation |
1) Department of Clinical Laboratory, Saga-Ken Medical Centre Koseikan 2) Department of Infection Control, Saga-Ken Medical Centre Koseikan 3) Department of Clinical Laboratory, Takagi Hospital 4) Medical Laboratory Science, Graduate School of Health and Welfare Sciences, International University of Health and Welfare Graduate School 5) Osaka Sales Office, West Area Sales Department, Diagnostics Sales Operations, Diagnostics Division, FUJIFILM Wako Pure Chemical Corporation 6) Application Department, Daltonics Divison, Bruker Japan K. K. 7) Department of Medical Technology and Sciences, School of Health Sciences at Fukuoka, International University of Health and Welfare |
| Publication | J.J.C.M.: 32 (1), 44-49, 2022 |
| Received | December 25, 2020 |
| Accepted | August 17, 2021 |
| Abstract | A 74 years-old male patient with pancreatic cancer who was received adjuvant chemotherapy experienced prolonged febrile symptom because of Citrobacter freundii bacteremia confirmed by blood culture. Although a matched antibiotic treatment was conducted after obtaining the report of the first blood culture, the patient did not show convalescence for more than 30 days and the second blood culture was indicated. An isolate, isolate #2 in this report, from the second blood culture exhibited biochemical properties resemble Shigella but identified as C. freundii complex by VITEK MS® (bioMérieux). Interestingly, isolate #2 gave rise small number of variant colonies when the BTB agar plate was incubated for extended period. This variant was picked up as isolate #3 and showed a stable phenotype of typical C. freundii as the strain from the first blood culture, isolate #1. These three isolates were indistinguishable by Pulse-field Gel Electrophoresis (PFGE) and IR Biotyper® (Bruker) Fourier Transform Infrared (FT-IR) analyses, both of which discriminate bacterial organisms at strain level. Consequently, the variant with Shigella-like reversible phenotype was thought to be originated from typical C. freundii strain within the patient's body under the pressure of host defense and antimicrobial treatment. |
| Keywords | Citrobacter freundii complex, PFGE |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |