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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Investigation of the prevalence of blaGES genes in Pseudomonas aeruginosa isolates |
| Language |
J |
| AuthorList |
Hirofumi Toda1), Shuichi Kubo1), Yuji Tanaka2), Toshinori Kamisako1,2), Koichiro Yoshida3) |
| Affiliation |
1) Department of Clinical Laboratory, Kindai University Hospital
2) Department of Clinical Laboratory Medicine, Kindai University Faculty of Medicine
3) Department of Medical Safety Administration, Division of Infection Control and Prevention, Kindai University Hospital |
| Publication |
J.J.C.M.: 32 (1), 57-60, 2022 |
| Received |
June 12, 2021 |
| Accepted |
July 30, 2021 |
| Abstract |
We investigated the prevalence of blaGES genes among 775 Pseudomonas aeruginosa clinical isolates. In this study, P. aeruginosa isolates resistant to ceftazidime (MIC ≥ 16 μ g/mL) were evaluated for the presence of genes encoding blaGES by PCR. Overall, 3 of 775 (0.4%) clinical isolates obtained from 2016 to 2018 harbored the blaGES gene. These three isolates were found to carry the blaGES-1 and blaGES-5 by genome sequencing. In MLST analysis, all of these isolates were of the same sequence type, ST235. In PCR-based open-reading frame (ORF) typing (POT) assay, these isolates showed the same POT number 205-58. This study revealed that the prevalence of blaGES-5 remains extremely low at 0.1% among P. aeruginosa isolates collected at our hospital located in the southern part of Osaka Prefecture. Because the blaGES genes may spread by horizontal transmission of plasmids between different bacterial species in clinical setting, monitoring trends for blaGES genes in diverse species is considered to be vital. |
| Keywords |
Pseudomonas aeruginosa, blaGES type, blaGES-5 |
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