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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Optimal CD toxin test conditions for C.DIFF QUIK CHEK COMPLETE using Clostridioides difficile colonies |
| Language |
J |
| AuthorList |
Yasushi Kibe1), Makiko Kiyosuke1), Taeko Hotta1), Dongchon Kang1,2) |
| Affiliation |
1) Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
2) Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences |
| Publication |
J.J.C.M.: 32 (4), 229-233, 2022 |
| Received |
March 31, 2022 |
| Accepted |
May 25, 2022 |
| Abstract |
Clostridioides difficile toxin analysis using C. difficile colonies in immunochromatography (colony IC) is important for the diagnosis of C. difficile infection (CDI) and is performed in many clinical laboratories. However, its standard procedure is not defined and various sample conditions are used. We evaluated the culture time and the concentration of the bacterial solution for colony IC to determine the optimal testing conditions. We used 30 strains of C. difficile which were isolated and stored at our hospital between January 2011 and December 2018. Their toxin genotypes were A+B+for 25 strains and A−B+for five strains. Colonies from these strains were cultured in Vital Media CCMA medium under anaerobic conditions at 35°C for 24, 48, and 72 hours. Then McFarland (McF) 2.0, 3.0, 4.0, 5.0, and 6.0 solutions were prepared for each colony. The solutions were analyzed for the toxins with C. DIFF QUIK CHEK COMPLETE. In the analysis using 24-hour culture solutions, 100% sensitivity was not obtained at any McFs. The conditions for 100% sensitivity were McF 5.0 or higher for 48-hour culture and McF 2.0 or higher for 72-hour culture. Therefore, from viewpoints of test speed and result accuracy, desired testing conditions were considered to be 48-hour culture with a McF of 5.0 or higher. This standardization of the sample preparation for colony IC can contribute to accurate CDI diagnosis. |
| Keywords |
Clostridioides difficile, toxigenic culture |
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