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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of DPS192iX EPG1 panel for antimicrobial susceptibility testing of β-lactamase-producing Enterobacterales and diagnostic accuracy of metallo-β-lactamase producers |
| Language |
J |
| AuthorList |
Sayaka Matsumoto1), Kenichiro Ohnuma1,2), Masako Nishida1), Mari Kusuki1,2), Nami Ishida1), Saori Kobayashi1), Keisuke Nishikawa1), Goh Ohji1,2,3), Takayuki Miyara2), Takamitsu Imanishi1), Yoshihiko Yano1,4) |
| Affiliation |
1) Department of Clinical Laboratory, Kobe University Hospital
2) Department of Infection Prevention and Control, Kobe University Hospital
3) Division of Infectious Diseases Therapeutics, Department of Microbiology and Infectious Diseases, Kobe University Graduate School of Medicine
4) Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine |
| Publication |
J.J.C.M.: 32 (4), 238-244, 2022 |
| Received |
March 29, 2022 |
| Accepted |
June 8, 2022 |
| Abstract |
The spread of carbapenemase-producing Enterobacterales (CPE) is a global threat to public health. Antimicrobial susceptibility testing (AST) results are necessary for optimal antibiotic management and infection control. In addition, because IMP-type metallo-β-lactamase (MBL) is a dominant type of carbapenemase in Japan, a simple and accurate method for detecting MBL producers is required. In this study, using 110 strains of β-lactamase-producing Enterobacterales (including 54 CPEs), we evaluated the performance of a DPS192iX EPG1 panel containing wells in which dipicolinic acid (DPA) was added and its accuracy in detecting MBL producers. The antimicrobial susceptibility of 54 CPEs and 56 non-CPEs was measured using EPG1 and compared with that of dry plate 'Eiken' (DP) as the reference method. The MIC essential agreements were 86.4%-100%, and the categorical agreements were 82.7%-100%. Furthermore, the detection rate of MBL producers defined as positive when the MICs of ceftazidime, imipenem, and meropenem containing DPA decreased by four times or more compared with that without DPA was 95.3%. Eleven CPEs except for MBL producers and 56 non-CPEs were determined as negative. Thus, AST using the DPS192iX system EPG1 panel may be useful for detecting MBL producers in clinical microbiology laboratories. |
| Keywords |
metallo-β-lactamase, DPS192iX |
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