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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of the antimicrobial susceptibility testing for Gram-negative organisms in positive blood culture specimens |
| Language |
J |
| AuthorList |
Kohei Uechi, Ami Takahashi, Maria Shimoji, Shohei Yogi, Ayumi Uechi, Shiro Maeda |
| Affiliation |
Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital |
| Publication |
J.J.C.M.: 32 (4), 245-251, 2022 |
| Received |
April 14, 2022 |
| Accepted |
July 19, 2022 |
| Abstract |
A rapid report for the antimicrobial susceptibility testing (AST) is required for the treatment of patients with bloodstream infections (BSI), but it takes more than 48 hours for obtaining the results of standard ASTs. In this study, we peroformed the D-ASTs using positive blood culture specimens to know the clinical usefulness of these rapid tests by evaluating concordance rates with the standard tests or required times for reporting their results. A total of 103 specimens, 28 blood samples inoculated with storage organisms (20 Gram-negative rods with/without known drug resistance genes, 8 ATCC strains), and 75 clinical specimens, were used for this study. Positive blood culture specimens were pretreated with erythrocyte lysis buffers and after centrifugation, pellets were dissolved in sterile saline, and applied to VITEKⓇ 2 for the AST. Regarding D-AST, rates of essential agreement within±1 tube difference, category agreement, very major error, and minor error were 97.8%, 97.5%, 0.5% and 2.0%, respectively. The required times were 10.9, 6.0, 18.7 hours, median, shortest and longest, respectively. These results indicate that the D-AST using VITEKⓇ 2 are rapid and accurate methods, and may contribute to the improvement of the treatment of patients with BSI by combining the antimicrobial stewardship program. |
| Keywords |
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