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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Performance evaluation of C. Diff selective multiplication broth medium without anaerobic culture |
| Language |
J |
| AuthorList |
Saori Ookawachi1), Tatsuya Natori1), Norikuni Uchida2), Eriko Arai1), Kazuki Horiuchi1) |
| Affiliation |
1) Department of Laboratory, Medicine Shinshu University Hospital
2) Life Science Laboratory, Technology & Development Division, Kanto Chemical Co., Inc. |
| Publication |
J.J.C.M.: 32 (4), 267-270, 2022 |
| Received |
March 9, 2022 |
| Accepted |
May 30, 2022 |
| Abstract |
The immunochromatographic (IC) method used in the diagnosis of Clostridioides difficile (C. difficile) infection (CDI) is a simple and rapid test, but has the problem of insufficient sensitivity in detecting toxins. C. Diff medium is a selective multiplication medium for C. difficile that does not require anaerobic incubation, and feces are incubated aerobically for 24-48 hours after inoculation, and those that turn yellow are considered positive, and the IC method can be used to detect toxin with high sensitivity. In this study of 100 fecal samples, sensitivity of toxin detected by the direct-IC method were 27.3%, by the C. Diff medium method were 36.4% in 24-hour incubation and 72.7% in 48-hour incubation. The sensitivity for C. difficile growth was 85.7% for both 24- and 48-hour cultures, and the specificity was 83.5% and 75.9%, respectively. The sensitivity of C. difficile growth was 85.7% for 24- and 48-hour incubations, and the specificity was 83.5% and 75.9% for 24- and 48-hour incubations, respectively. Since nonspecific reactions were also observed, it was considered necessary to use the C. Diff-IC method in combination with the color change of the medium. Since C. Diff medium can be used to culture C. difficile under aerobic conditions, it may be useful in facilities that do not have facilities for anaerobic culture or genetic testing, and where testing for CDI is limited to the IC method. |
| Keywords |
Clostridioides difficile, CDI |
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