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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Repeat copy number changes in MLVA of Enterohemorrhagic Escherichia coli isolated multiple times from the same patient |
| Language |
J |
| AuthorList |
Takashi Sato, Moeko Muta, Nobuko Tsukamoto, Takayuki Kurazono, Hirokazu Fukushima, Tsuyoshi Kishimoto, Asao Honda |
| Affiliation |
Saitama Institute of Public Health |
| Publication |
J.J.C.M.: 33 (1), 12-17, 2022 |
| Received |
May 15, 2022 |
| Accepted |
August 10, 2022 |
| Abstract |
Multiple-locus variable-number tandem repeat analysis (MLVA), a molecular epidemiological analysis method for enterohemorrhagic E. coli, is effective in identifying the extent of outbreak cases and detecting diffuse outbreaks. This study focused on changes in MLVA repeat sequences in the same patient organism using clinical isolates derived from patient stools, including asymptomatic pathogen carriers. We performed MLVA analysis on 202 isolates (61 individuals) isolated multiple times from the same infected person between 2015 and 2018 to see changes in MLVA patterns between first and second and subsequent isolates from the same infected person. The results showed that 7 of 31 (22.6%) of the O157 isolates and 8 of 30 (26.7%) of the O26 isolates showed a change from the MLVA pattern of the initial isolates. In addition, 12 colonies per isolate were caught and analyzed by MLVA for stool-derived strains from 3 patients with O157 and two patients with O26 who had been isolated five or more times. The results showed that the first pattern change was observed in the second isolates after 5 to 8 days from the initial isolation. Artificial passaging was performed using the first isolates of the three O157 strains that showed this pattern change, and the pattern change was observed only after the 40th or later subcultures. No clear relationship between MLVA pattern change and drug use history could be confirmed in this study. |
| Keywords |
MLVA |
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