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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Clinical evaluation of LIMSAVE MT-7500 for measurement of plasma (1→3)-β-D-glucan and endotoxin |
| Language |
J |
| AuthorList |
Keiichiro Mori1), Yasufumi Matsumura1,2,3), Yasuhiro Tsuchido1,2,3), Satomi Yukawa1,2,3), Taro Noguchi1,2,3), Masaki Yamamoto1,2,3), Miki Nagao1,2,3) |
| Affiliation |
1) Department of Clinical Laboratory, Kyoto University Hospital
2) Department of Infection Control and Prevention, Kyoto University Hospital
3) Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine |
| Publication |
J.J.C.M.: 33 (1), 18-24, 2022 |
| Received |
June 14, 2022 |
| Accepted |
August 12, 2022 |
| Abstract |
(1→3)-β-D-glucan (BDG) and endotoxin are used for rapid adjunctive diagnosis of invasive fungal diseases and for severe gram-negative bacilli infections, respectively. FUJIFILM Wako Pure Chemical Corporation has developed a new measurement device for BDG and endotoxin, LIMSAVE MT-7500. In this study, we compared measurement values of clinical samples measured by MT-7500 and TOXINOMETER MT-6500 using the same Limulus Amebocyte Lysate assay reagents. Between December 2020 to July 2021, plasma samples drawn for BDG or endotoxin measurement at Kyoto University Hospital were included. Correlation of measured values and concordance of qualitative results were calculated using 1076 plasma samples for BDG and 178 plasma samples for endotoxin. The correlation coefficient of the BDG values between two devices was 0.996, and the positive agreement rate, negative agreement rate, and overall agreement rate were respectively 93.9%, 99.8%, and 98.9% at a cutoff value of 11 pg/mL. The correlation coefficient of endotoxin values between the two devices was 0.970, and the positive agreement rate, negative agreement rate, and overall agreement rate were all 100% at a cutoff value of 5.0 pg/mL. MT-7500 presented a similar diagnostic performance to MT-6500 in measurement of BDG and endotoxin. |
| Keywords |
(1→3)-β-D-glucan, invasive fungal diseases, endotoxin, sepsis, endotoxemia |
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