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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Genetic detection of oral bacteria using tablet medium |
| Language |
J |
| AuthorList |
Kentaro Nagamine1), Rumi Kimura1), Masae Kitagawa2) |
| Affiliation |
1) Department of Clinical Nutrition, Faculty of Health and Wellness Science, Hiroshima International University
2) Department of Oral Maxillofacial Pathobiology, Graduate School of Biomedical and Health Sciences, Hiroshima University |
| Publication |
J.J.C.M.: 33 (1), 25-29, 2022 |
| Received |
August 1, 2022 |
| Accepted |
August 31, 2022 |
| Abstract |
Some oral bacteria are involved in systemic diseases as well as oral diseases such as dental caries and periodontal disease. Detection and identification of these oral bacteria from saliva requires culture of the bacteria, DNA extraction from the bacteria, and confirmation by PCR. In this study, we aimed to simplify the operation from saliva sampling to detection by improving the saliva culture method and combining it with the loop-mediated isothermal amplification (LAMP) method. A tablet-shaped solid medium, named tablet medium, and saliva sampled from the oral cavity are mixed in a microtube and left in a constant temperature bath at 37°C for one day, and as a result, Streptococcus mutans and Streptococcus sobrinus were able to grow. The next day, S. mutans and S. sobrinus could be amplified directly from the cultured saliva for LAMP reaction without DNA extraction. This method is suitable for processing a large number of samples easily and quickly, and is expected to be used for general-purpose detection of oral bacteria in hospitals, dental clinics, and group examinations. |
| Keywords |
tablet medium, loop-mediated isothermal amplification, oral bacteria, cavity, genetic diagnosis |
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