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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Investigation of the necessity of PBP2´ detection in Coagulase negative Staphylococcus with Oxacillin MIC of 0.5 to 2 μg/mL |
| Language |
J |
| AuthorList |
Erika Nakazato, Yumiko Kimura, Kazumasa Sasaki, Fuminori Abe |
| Affiliation |
Department of Clinical Laboratory, Jichi Medical University Hospital |
| Publication |
J.J.C.M.: 33 (1), 30-35, 2022 |
| Received |
May 5, 2022 |
| Accepted |
September 5, 2022 |
| Abstract |
The CLSI M100-30th Edition recommended in 2020, Staphylococcus spp. are classified and described into four groups according to the conditions of oxacillin (MPIPC) and cefoxitin assay. Among them, MPIPC MIC values of 0.5 to 2 μg/mL for "Other Staphylococcus spp." may over determine methicillin resistance, and in severe infections, detection of mecA gene or Penicillin binding protein2´ is necessary.
We performed mecA PCR detection and PBP2´ latex agglutination test using 30 strains of "Other Staphylococcus spp." isolated at our hospital with MIC values of 0.5 to 2 μg/mL for MPIPC. The results showed that 17 isolates (56.7%) were negative for both tests, indicating that more than half of the isolates were over determined for methicillin resistance. Therefore, we believe that the detection of PBP2´ by latex agglutination test can more conveniently determine methicillin resistance and enable more appropriate early selection of therapeutic agents. |
| Keywords |
PBP2´, Other Staphylococcus spp., oxacillin |
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