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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.33 No.1 contents] Japanese / English |
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| ArticleTitle | Molecular characterization of Linezolid non susceptibility enterococci isolated from secondary healthcare hospital in Tokyo, 2013-2020 |
| Language | J |
| AuthorList | Yukitaka Ito1,2), Takahiro Nomura2), Koichi Tanimoto3), Naomi Kaneko1), Mieko Yoshida1), Masanobu Otsuka1), Haruo Nakayama4), Hiroto Matsuse4), Haruyoshi Tomita2,3) |
| Affiliation |
1) Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center 2) Department of Bacteriology, Gunma University Graduate School of Medicine 3) Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine 4) Department of Infection and Prevention, Toho University Ohashi Medical Center |
| Publication | J.J.C.M.: 33 (1), 36-43, 2022 |
| Received | July 29, 2022 |
| Accepted | September 26, 2022 |
| Abstract |
We performed a molecular genetic characterization of Linezolid non susceptibility enterococci (LNSE) isolated from January 2013 to December 2020 in secondary healthcare hospital in Tokyo, Japan. Four LNSE (0.002%) were detected from 2, 191 clinical isolates of enterococci. LNSE were examined to have resistance genes, namely optrA, cfr, poxtA, by PCR, and mutations in 23S rRNA gene and ribosomal protein genes, such as L3 (rplC), L4 (rplD), and L22 (rplV) by DNA sequencing. TOH-EF1 had a base substitution (A2504T) in 23S rRNA gene and TOH-1411 had a base substitution (G2576T) in 23S rRNA gene. In addition, TOH-EF9 and TOH-588 were found to have optrA on a plasmid as well as fexA conferring florfenicol and chloramphenicol resistance to the host. The optrA-carrying plasmid of TOH-EF9 was transferred to E. faecalis FA2-2 in broth at a high frequency. PFGE revealed no genetic relationship between the 4 LNSE, which were classified as different STs (ST17, ST480, ST234, and ST179) by MLST. In conclusion, LNSE harboring optrA encoded on a mobile genetic factor (conjugative plasmid) may spread rapidly under drug pressure, so we should take precautions. There is also concern that the effectiveness of LZD treatment may decrease in the future. |
| Keywords | Linezolid, enterococci, 23S rRNA, optrA |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |