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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of the usefulness of reagents for detecting Clostridioides difficile Toxin in a 3-step algorithmic method |
| Language |
J |
| AuthorList |
Mikiko Chiba, Asami Nakayama, Yuriko Miura, Makoto Katsumi, Yuko Abe, Shin-ichi Fujimaki, Toru Fujiwara |
| Affiliation |
Department of Clinical Laboratory, Tohoku University Hospital |
| Publication |
J.J.C.M.: 33 (2), 109-115, 2023 |
| Received |
July 31, 2022 |
| Accepted |
November 25, 2022 |
| Abstract |
In order to evaluate the potential usefulness of 3-step algorism-based Clostridioides difficile Toxin detection3), we compared conventional reagent (C. DIFF QUIK CHEK COMPLETE [CHEK COMPLETE]; Kozin Bio., Japan) with the newer reagent (Quick Chaser CD GDH/TOX [QUICK CHASER]; Mizuho Med., Japan). In the evaluation with stored strains, CHEK COMPLETE showed higher detection sensitivity for both GDH and Toxin than QUICK CHASER. However, the detection sensitivity for Toxin was affected by the ribotypes; CHEK COMPLETE and QUICK CHASER showed lower detection sensitivity against ribotypes 014 and 014/369, respectively. In the evaluation with patient stool samples, the sensitivity/specificity for CHEK COMPLETE and QUICK CHASER were 100.0%/96.3% and 89.5%/100.0% (GDH), 46.7%/100.0% and 26.7%/100.0% (Toxin), respectively. Based on our results, CHEK COMPLETE exhibits sufficient quality in 3-step algorism-based Clostridioides difficile Toxin detection, while there remains a concern regarding the high cost of the reagent. On the other hand, QUICK CHASER is easy to use, requiring only 15 minutes per step, while showing lower detection sensitivity for GDH than CHEK COMPLETE. In addition, when QUICK CHASER is used by Toxigenic culture method3), it would be necessary to aware that the approach could show lower detection sensitivity for some strains according to the ribotypes. |
| Keywords |
CDtoxin, C. difficile, GDH, Ribotype |
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