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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Comparison of CHROMagar Candida-based estimation and MALDI-TOF MS-based identification of Candida species detected in blood cultures |
| Language |
J |
| AuthorList |
Osanori Nagura1), Kazuki Furuhashi1), Yuki Nagao1), Yusuke Ota1,2), Jinko Ishikawa1), Masato Maekawa1,3) |
| Affiliation |
1) Department of Clinical Laboratory, Hamamatsu University Hospital
2) Department of Molecular Microbiology, Tokyo Medical and Dental University
3) Department of Laboratory Medicine, Hamamatsu University School of Medicine |
| Publication |
J.J.C.M.: 33 (2), 116-120, 2023 |
| Received |
May 26, 2022 |
| Accepted |
December 12, 2022 |
| Abstract |
Candida species are resident in the human intestinal tract and other parts of the body. In Candidemia, rapid identification of Candida species is important, because delayed diagnosis and treatment worsen prognosis. We identified Candida species detected in the blood cultures for past twelve years using Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and compared the estimation using chromogenic substrate media. One hundred twenty-five of Candida species were detected from blood cultures. In MALDI-TOF MS-based identification, Candida albicans is the most frequently detected, followed by Candida parapsilosis, Candida glabrata, and Candida tropicalis. Additionally, Candida guilliermondii, Candida dubliniensis, Candida orthopsilosis, Candida lusitaniae, and Lodderomyces elongisporus were identifiable. On the other hand, seventeen estimation results of using chromogenic substrate media were inconsistent with the identification using MALDI-TOF MS. From the results of sequence analysis, MALDI-TOF MS for identification of Candida species proved to be rapid and accurate. In Candidemia, rapid identification of Candida species is essential to the selection of antifungal agents and determination of treatment strategy. We suppose that identification of Candida species detected from the blood cultures using MALDI-TOF MS contributes to them. |
| Keywords |
MALDI-TOF MS |
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