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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Isolation frequency and chronological change of non-tuberculosis mycobacteria in Kawasaki Municipal Ida Hospital |
| Language |
J |
| AuthorList |
Yuki Sekine1), Hitomi Kikuchi1), Kenta Sasaki1), Shuhei Kobanawa1), Satoshi Mitarai2), Kazumi Nishio3) |
| Affiliation |
1) Department of Laboratory, Kawasaki Municipal Ida Hospital
2) Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association
3) Department of Respiratory Medicine, Kawasaki Municipal Ida Hospital |
| Publication |
J.J.C.M.: 33 (3), 196-200, 2023 |
| Received |
December 14, 2022 |
| Accepted |
February 7, 2023 |
| Abstract |
Background: The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) enables detailed identification of bacterial species, notably difficult to identify mycobacterial species. Purpose: To assess the detectability of non-tuberculosis mycobacteria (NTM) at Kawasaki Municipal Ida Hospital from 2012 to 2021, and clarify the detection status and transition of NTM. Results: Total 854 NTM isolates were isolated from clinical specimens during the study period, among which Mycobacterium avium/intracellulare complex (MAC, 528 isolates) accounted for 61.8% of the total isolates, followed by Mycobacterium gordonae (118 isolates, 13.8%), Mycobacterium abscessus species (40 isolates, 4.7%), and Mycobacterium kansasii (40 isolates, 4.7%). During the period of investigation, the identification method was changed from the DNA-DNA hybridization to MALDI-TOF MS, which increased the number of bacterial species identified, including the rare bacterial species such as Mycobacterium paragordonae, Mycobacterium lentiflavum, and Mycobacterium mucogenicum. Discussion: MALDI-TOF MS enables the identification of rare bacterial strains, and thus, can make important contributions in clinical settings, such as facilitating the selection of effective drugs. |
| Keywords |
MALDI-TOF MS |
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