 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
A case of vertebral osteomyelitis caused by Veillonella parvula, identified using broad-range polymerase chain reaction |
| Language |
J |
| AuthorList |
Motoki Fujii1), Naoto Hosokawa1), Yoshifumi Kubota1), Yu Niiyama2), Tomohisa Watari3), Yoshihito Otsuka3) |
| Affiliation |
1) Department of Infectious Diseases, Kameda Medical Center
2) Department of General Medicine, Matsuyama Red Cross Hospital
3) Department of Laboratory Medicine, Kameda Medical Center |
| Publication |
J.J.C.M.: 33 (4), 290-295, 2023 |
| Received |
April 5, 2023 |
| Accepted |
June 8, 2023 |
| Abstract |
Broad-Range PCR assay for detection of the 16S rRNA gene is a method for detecting bacteria by amplifying the 16S rRNA region common to bacteria from specimens. We report a case of culture-negative vertebral osteomyelitis in which the organism was identified by 16S rRNA gene analysis of a biopsy specimen. A 78-year-old woman presented to our hospital with complaints of back pain and motor paralysis of both lower limbs. Thoracic spine Magnetic Resonance Imaging scan revealed Th9-Th11 vertebral osteomyelitis. On the day of admission, the patient developed a fever, and Escherichia coli was confirmed in her blood and urine cultures. The patient was diagnosed with acute pyelonephritis, and ceftriaxone (CTRX) treatment was initiated. A biopsy of the vertebral body was performed after CTRX administration, but the culture was negative. A 16S rRNA analysis of the vertebral body specimen by broad-range PCR detected Veillonella parvula. We assumed that V. parvula, which induced vertebral osteomyelitis, originated from her poor oral hygiene. This case suggests that 16S rRNA analysis of specimens by broad-range PCR may be useful when the causative pathogen cannot be identified by conventional culture because of prior exposure to antimicrobial agents which are difficult to discontinue. |
| Keywords |
Veillonella parvula, Broad-range PCR |
|
