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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Detection of decreased permeability in Enterobacterales with reduced susceptibility to carbapenem using polymyxin B nonapeptide |
| Language |
J |
| AuthorList |
Masamune Aihara1,2), Yukako Yanagisawa1), Rikako Nishi3), Yuka Yamashita1), Yasushi Kibe1), Makiko Kiyosuke1), Taeko Hotta1) |
| Affiliation |
1) Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
2) Department of Health Science, Graduate School of Medical Sciences, Kyushu University
3) Department of Laboratory Medicine, Hospital of The University of Occupational and Environmental Health |
| Publication |
J.J.C.M.: 33 (4), 296-301, 2023 |
| Received |
April 6, 2023 |
| Accepted |
June 9, 2023 |
| Abstract |
Most carbapenem-resistant Enterobacterales (CRE) in Japan would acquire resistance to carbapenems through decreased permeation of antimicrobials. However, since there are no clinical tests available to detect drug permeabilities, the mechanisms of decreased susceptibility to carbapenem in clinical isolates remain fully unexplored. We focused on polymyxin B nonapeptide (PMBN), which is known to increase in membrane permeability, and found that the meropenem MICs of CRE were reduced by up to 32-fold in the presence of 128 μg/mL PMBN. By using this condition, we demonstrated that significantly more isolates in the modified carbapenem inactivation method (mCIM)-negative CRE group had decreased meropenem MIC in the presence of PMBN than in the mCIM-positive CRE group. This method could potentially facilitate the detection of decreased permeabilities in Enterobacterales with reduced susceptibility to carbapenem. |
| Keywords |
CRE, CPE, polymyxin B nonapeptide |
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