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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Verification of discrepancies and factors in susceptibility of ESBL-producing Escherichia coli using automatic susceptibility measurement equipment |
| Language |
J |
| AuthorList |
Naoya Ogura, Haruka Yokoo, Wakaba Kurogi, Ayako Nakamura |
| Affiliation |
Department of Clinical Laboratory, Juntendo Tokyo Koto Geriatric Medical Center |
| Publication |
J.J.C.M.: 34 (1), 49-53, 2023 |
| Received |
June 3, 2023 |
| Accepted |
August 17, 2023 |
| Abstract |
We examined the factors that led to the higher susceptibility rate of Cefepime (CFPM) compared to other reports following the change in the drug susceptibility measurement panel. The MIC concordance rates of NM-EN2J (NE), Plate RCEB1 (PR), and NMIC/ID-441 (NI) based on Dry Plate 41 (DP) in 15 ESBL-producing E. coli strains were 87% or higher for the five major agents. For CFPM, the concordance rates for PR, NI, and NE were 80%, 53%, and 20%, respectively, with NE being particularly low at 33%, even with a ±1 dilution difference. Comparing the disk diffusion method of CFPM with the MICs of each panel, the categories of DP, PR, and NI were almost identical, while 11 isolates of NE were discordant. Significant differences were also observed for the divalent ions and electrolytes in the wells. The MIC of CFPM tended to be higher in the NE. The reason may be due to the components of the culture medium. However, there are many factors that can change the MIC, and pharmacological and bacteriological validation is also needed. |
| Keywords |
Cefepime (CFPM), Escherichia coli |
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