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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.34 No.2 contents] Japanese / English |
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| ArticleTitle | Effect on Gram staining at water temperature or water quality |
| Language | J |
| AuthorList | Kyohei Wada1), Setsuko Kubo1), Yuzo Tsuyuki2), Sachiko Shibata1) |
| Affiliation |
1) Inspection Engineering Department, Sanritsu Co., Ltd. 2) Sanritsu Service Co., Ltd. |
| Publication | J.J.C.M.: 34 (2), 102-109, 2024 |
| Received | May 24, 2023 |
| Accepted | December 4, 2023 |
| Abstract |
Rapid confirmation of pathogenic bacteria is important in bacterial infections. Clinical microbiological examinations generally consist of smear staining, culture and susceptibility testing. Of these, culture and susceptibility testing usually take 2-3days and have disadvantage in term of rapid diagnosis. On the other hand, the smear staining test is simple in procedure and excellent in quick reporting. The Gram stain method is the most common procedure of the smear staining tests for pathogens. It allows rough estimation of bacterial species and tissue damage by stainability and morphological observations. The findings obtained can provide useful information for initial treatment of infectious diseases. We experienced the decrease of stainability in our laboratory this summer. Good staining results were obtained by using ion exchanged water containing no impurities as washing water. Therefore, it was suggested that the cause of the staining decline was water quality. To find out the cause, we investigated on the effects of the water quality used for rinsing. Assuming routine examinations, water temperature ranging from 5 to 30°C and water quality ranging from 0 to 304 mg/L in hardness were used in this study. As a result, the stainability was the best when rinse water at a water temperature of 5°C and a hardness of 0 mg/L was used, and the rinse water with a water temperature of 30°C and a hardness of 304 mg/L was the worst. It showed good stainability as the water temperature and hardness decreased. In this study, the Gram stainability was examined in terms of the temperature and hardness of rinse water. Although the effects of other factors cannot be ruled out, standardization of rinse water in Gram stain was considered necessary. |
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