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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of the clinical usefulness of the colistin broth disk elution as an antimicrobial susceptibility testing for colistin in Enterobacterales |
| Language |
J |
| AuthorList |
Ami Nakano1), Kohei Uechi1), Syohei Yogi2), Takaaki Tome1), Maria Nakamoto1), Shiro Maeda1,3) |
| Affiliation |
1) Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital
2) Division of Morphological Pathology, Department of Basic Laboratory Science, Faculty of Medicine, University of the Ryukyus
3) Department of Advanced Genomic and Laboratory Medicine, Graduate School of Medicine, University of the Ryukyus |
| Publication |
J.J.C.M.: 34 (3), 178-183, 2024 |
| Received |
December 15, 2023 |
| Accepted |
March 18, 2024 |
| Abstract |
A rapid and accurate antimicrobial susceptibility testing (AST) method is required in the antimicrobial stewardship activity to detect antimicrobial resistant bacteria as soon as possible. In this study, to evaluate the clinical usefulness of colistin broth disk elution (CBDE), an AST for colistin (CL), we examined seventy-seven isolates of Enterobacterales from various clinical specimens submitted between January 2016 and December 2019, including 4 isolates of E. coli harboring mcr-1 and 2 isolates of E. cloacae complex harboring mcr-9, with CBDE. Twenty-eight (36.4%) and 49 (63.6%) isolates were determined as intermediate, and resistant, respectively, and by using the broth microdilution (BMD) as a reference method, the category agreement (CA) and essential agreement (EA) of the MIC of CL within±1 tube difference are 93.5% (72/77 isolates) and 100% (77/77 isolates), respectively, we also observed 15.2% (5/33 isolates) of major error, but no very major error. Regarding six isolates harboring the mcr (4 isolates of E. coli harboring mcr-1 and 2 isolates of E. cloacae complex harboring mcr-9), four were determined as intermediate and two were resistant in BMD, whereas all six isolates were determined as resistant in CBDE. These results indicate that CBDE is considered as an accurate and rapid method to detect the CL-resistant Enterobacterales., and useful to apply antimicrobial stewardship activity for Enterobacterales. |
| Keywords |
Enterobacterales, Colistin：CL, Broth microdilution method（BMD）, Colistin Broth Disk Elution（CBDE） |
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