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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of Enterobacter cloacae complex identification performance by MALDI Biotyper and VITEK MS |
| Language |
J |
| AuthorList |
Shoya Kato1), Mika Miura1), Miyu Sato1), Mizuki Nakaya1), Kanon Takeda1), Naoki Wada1), Shoji Yashita2), Manabu Akiya2), Mizuki Matsunaga3), Maki Mochizuki4), Masaaki Shinagawa4) |
| Affiliation |
1) Department of Clinical Laboratory, Sapporo Tokushukai Hospital
2) Sapporo Higashi Tokushukai Hospital Laboratory Center
3) Clinical Laboratory, Department of Medical Technology, Mikasa Municipal General Hospital
4) Department of Clinical Laboratory Medicine, Faculty of Health Sciences, Japan Healthcare University |
| Publication |
J.J.C.M.: 34 (4), 267-272, 2024 |
| Received |
March 21, 2024 |
| Accepted |
June 17, 2024 |
| Abstract |
Enterobacter cloacae complex includes E. cloacae, E. hormaechei, and E. asburiae as major species, which are genetically similar and difficult to classify by biochemical identification and MALDI-TOF MS. With the update of the MALDI Biotyper library (Ver. 12.0.0.0, MSP number 11897), the number of registered strains of Enterobacter spp. has increased significantly, but there are no reports on the validation of identification performance. In this study, we evaluated the identification performance of the two models by comparing the identification results by MALDI Biotyper and VITEK MS for 72 strains isolated as E. cloacae complex from clinical materials from September to November 2022, based on the identification results by ANI analysis. Identification at rank 1 by MALDI Biotyper was matched at the species level for 57 of 72 (79.2%) of the isolates when compared to identification by ANI analysis. On the other hand, 70 of the 72 isolates (97.2%) had a score value of 2.00 or higher and contained the same species as the ANI analysis within rank 10. No single strain was identified as a single species in VITEK MS, and 23 (31.9%) of the total 72 strains for which multiple species were identified contained species that matched the ANI analysis, a low concordance rate. MALDI Biotyper did not identify any other Enterobacter spp. in the top 10 for E. hormaechei and E. bugandensis, suggesting that species names can be reported. In VITEK MS, all Enterobacter spp should be reported as E. cloacae complex, even if identified. |
| Keywords |
Enterobacter cloacae complex, MALDI Biotyper, VITEK MS |
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