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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Development and evaluation of a new phenotypic method for detection of extended-spectrum-, AmpC- and/or metallo-β-lactamase producing Enterobacterales |
| Language |
J |
| AuthorList |
Mina Takayama1), Daisuke Sakanashi1), Yuzuka Kawamoto1), Narimi Miyazaki1), Tomoko Ohno1), Atsuko Yamada1), Akiko Nakamura1), Hirotoshi Ohta1), Hiroyuki Suematsu1), Chida Sumie2), Jun Hirai1,2), Nobuhiro Asai1,2), Nobuaki Mori1,2), Hiroshige Mikamo1,2) |
| Affiliation |
1) Department of Infection Control and Prevention, Aichi Medical University Hospital
2) Department of Clinical Infectious Diseases, Aichi Medical University Hospital |
| Publication |
J.J.C.M.: 34 (4), 279-285, 2024 |
| Received |
March 21, 2024 |
| Accepted |
July 4, 2024 |
| Abstract |
Efficient detection of extended-spectrum β-lactamase (ESBL), AmpC β-lactamase (AmpC), and metallo β-lactamase (MBL) is a challenge in clinical laboratories. In this study, we developed and evaluated MastAE-SMA, a new method for detecting the β-lactamases, by combining MASTDISCS Combi AmpC and EsβL ID set (MastAE) and sodium mercaptoacetic acid double disk synergy test (SMA-DDST). MastAE-SMA, MastAE, and SMA-DDST were used to test 63 phenotypically and genotypically well-characterized Enterobacterales clinical isolates and seven reference strains (including two ESBL, 16 AmpC, 10 ESBL/AmpC, 13 MBL, 21 ESBL/MBL, six AmpC/MBL, and two ESBL/AmpC/MBL), and the results were compared with PCR results. MastAE and MastAE-SMA showed 100% (28/28) agreement with the PCR results for ESBL- and AmpC-producers, and 7% (2/29) and 66% (19/29) agreement, respectively, for MBL and ESBL- and/or AmpC producers. For MBL detection, SMA-DDST and MastAE-SMA showed 88% (37/42) agreement with PCR results. In conclusion, MastAE-SMA is an effective method for more accurately differentiating β-lactamases in MBL and ESBL and/or AmpC-producing bacteria. |
| Keywords |
SMA-DDST, β-lactamase |
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