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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.34 No.4 contents] Japanese / English |
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| ArticleTitle | Bartonella henselae bacteremia and infection-related glomerulonephritis detected by a blind subculture of blood culture media |
| Language | J |
| AuthorList | Kenjiro Matsui1,3), Toshiyuki Narimiya2), Hiroki Hayashi2), Kentaro Tsukamoto3,4), Aki Sakurai3,5), Yohei Doi3,5,6), Naotake Tsuboi2) |
| Affiliation |
1) Department of Clinical Laboratory, Fujita Health University Hospital 2) Department of Nephrology, Fujita Health University School of Medicine 3) Department of Microbiology, Fujita Health University School of Medicine 4) International Research Center for Infectious Diseases Laboratory of Bacterial Zoonoses, Research Institute for Microbial Diseases, Osaka University 5) Department of Infectious Diseases, Fujita Health University School of Medicine 6) Center for Infectious Disease Research, Fujita Health University |
| Publication | J.J.C.M.: 34 (4), 298-302, 2024 |
| Received | May 15, 2024 |
| Accepted | June 28, 2024 |
| Abstract | Bartonella species, which can cause blood stream infections including infective endocarditis, are rarely isolated from clinical specimens. Here, we present a 56-year-old male with a history of hypertension and thoracic aortic dissection who was hospitalized for prolonged fever and worsening renal function. Given the history of cat exposure and flea bites prior to the onset of symptoms, Bartonella infection was suspected. Therefore, blood culture specimens incubated on BACTEC FX Blood Culture System were subjected to blind subculture (i.e., subculture in the absence of detectable growth) and extended incubation. Blind subculture on chocolate agar plate was conducted on day 7 of incubation, which yielded small gray-colored colonies on day 13. These colonies were identified as Bartonella henselae based on sequencing of the ssrA and rpoB genes. Blood culture bottles did not flag positive by 3 weeks of incubation. Blind subculture of blood culture media might be useful for detecting Bartonella spp. from clinical specimens. |
| Keywords | Bartonella henselae |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |