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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Comparison of LAMP, FilmArray, and R Panel for detecting of Bordetella pertussis based on 4Plex real-time PCR and culture |
| Language |
J |
| AuthorList |
Shoko Komatsu, Tomoka Iwamoto, Noriko Nakanishi |
| Affiliation |
Health Research Department 2, Kobe Institute of Health |
| Publication |
J.J.C.M.: 36 (1), 38-42, 2025 |
| Received |
October 8, 2025 |
| Accepted |
November 5, 2025 |
| Abstract |
In 2025, pertussis is spreading nationwide in Japan. We compared the detection capabilities of loop-mediated isothermal amplification (LAMP), BioFire FilmArray Respiratory Panel 2.1 (FilmArray), and BioFire SpotFire R Panel (R Panel), which are primary diagnostic tests for Bordetella pertussis, using 4Plex real-time PCR Ct values and culture results. For nasopharyngeal specimens with Ct values of >30.0, the R Panel showed higher positive rates than the other tests, whereas the FilmArray exhibited a lower rate. When comparing LAMP and FilmArray, both targeting the B. pertussis-specific gene ptxP, LAMP showed a higher positive rate within the same Ct value range, and some specimens that were negative using FilmArray were positive using LAMP. Among specimens positive by the LAMP/FilmArray/R Panel, culture positively was approximately 80%. Although culture positivity decreased with increasing Ct values, specimens with a Ct value of 37 were still culture-positive, indicating that high Ct values do not necessarily correspond to negativity. Our findings indicate that understanding the characteristics of each diagnostic test and selecting the appropriate test according to the clinical context is critical. |
| Keywords |
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