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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.36 No.2 contents] Japanese / English |
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| ArticleTitle | False negatives in FilmArrayⓇ caused by Haemophilus influenzae hpd gene deletion |
| Language | J |
| AuthorList | Mikako Morinaga1), Yusuke Isobe2), Michiko Miyahara1), Tadashi Inoue1), Tomoyuki Tame1), Shunpei Kurosaki3,4), Meiwa Shibata1,2), Shunsuke Segawa5), Akiko Miyabe5), Misako Ohkusu6), Mai Kagawa7), Masahiro Oda7), Akihiro Bessho7, 8), Naruhiko Ishiwada6), Yuho Horikoshi2) |
| Affiliation |
1) Department of Clinical Laboratory, Tokyo Metropolitan Children's Medical Center 2) Division of Infectious Diseases, Department of Pediatrics, Tokyo Metropolitan Children's Medical Center 3) National Institute of Neuroscience, National Center of Neurology and Psychiatry 4) Graduate School of Medical and Dental Sciences, Institute of Science Tokyo 5) Division of Laboratory Medicine, Chiba University Hospital 6) Department of Infectious Diseases, Medical Mycology Research Center, Chiba University 7) Department of Clinical Laboratory, Japanese Red Cross Okayama Hospital 8) Department of Respiratory Medicine, Japanese Red Cross Okayama Hospital |
| Publication | J.J.C.M.: 36 (2), 132-136, 2026 |
| Received | May 15, 2025 |
| Accepted | December 15, 2025 |
| Abstract | We experienced a Haemophilus influenzae bacteremia that is false negative by the FilmArray (BioFire Diagnostics) Blood Culture Panel 2. Genetic analysis revealed a deletion in the Haemophilus Protein D (hpd) gene. Notably, four additional strains harboring the same mutation were also negative for H. influenzae on the FilmArray Blood Culture Identification 2 Panel. These findings suggest that the observed mutation in the hpd gene may interfere with detection by FilmArray panels. Although multiplex PCR-based diagnostic tools such as the FilmArray offer rapid and user-friendly identification of pathogens, discrepancies with conventional methods necessitate careful interpretation. In cases of discordant results, the possibility of false negatives should be considered, particularly when genetic mutations may affect primer or probe binding sites. It is important to interpret the results through discussion between laboratory technicians and clinicians, based on the Gram stain findings and the patient's clinical course. |
| Keywords | Haemophilus influenzae, FilmArrayⓇ |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |