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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Comprehensive Evaluation of a Line Probe Assay Kit for Rapid Detection of Rifampicin-Resistant Mycobacterium tuberculosis: Clinical Performance and Inference of Infection Occurrences |
| Language |
J |
| AuthorList |
Shiomi Yoshida1),*, Motohisa Tomita2), Mika Kihara2), Hiroyuki Kikkawa3) |
| Affiliation |
1) Clinical Research Center, 2) Department of Clinical Laboratory, National Hospital Organization Kinki-chuo
Chest Medical Center, 1180 Nagasone-cho, Kita-ku Sakai, Osaka 591-8555; *Corresponding author
3) Department of Clinical Laboratory, National Hospital Organization Toneyama Hospital,
5-1-1 Toneyama, Toyonaka, Osaka 560-8552 |
| Publication |
J.J.C.M.: 22 (1), 42-48, 2012 |
| Received |
August 3, 2011 |
| Accepted |
December 24, 2011 |
| Abstract |
We evaluated a line probe assay (LiPA) kit, which was designed to identify Mycobacterium tuberculosis complex, and to detect mutations related to resistance to rifampicin for rapid detection of MDR-TB. A total of 750 clinical specimens from pulmonary and extrapulmonary sites were tested with LiPA directly and drug-susceptibility testing. Sequence in rpoB gene was also performed to 27 clinical isolates with both isoniazid- and rifampicin-resistance, and the LiPA could detect rifampicin-resistant TB in 35 specimens. Compared with results of LiPA, drug susceptibility testing and rpoB sequence, several discordant results were observed in 2 rifampicin mono-resistance TB strains and 2 MDR-TB strains with rpoB mutations. The LiPA was more than just useful for the detection of MDR-TB from variable clinical specimens, although the LiPA caused rare nonspecific reactions as the appropriate situations or correct operations. It may provide clinical managements and summarize the evidence of molecular epidemiology of rifampicin-resistant TB. |
| Keywords |
Line probe assay, MDR-TB |
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