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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Pseudo-outbreak of Stenotrophomonas maltophilia Determined by Molecular Typing |
| Language |
J |
| AuthorList |
Kentaro Kikuchi1), Suzuko Ashikawa1), Mai Otuda1), Masako Tajima1), Atsushi Ichinose1), Isao Shibuya1), Hiroyuki Mushiake1), Chiyoko Motegi1), Takehiro Asada1), Hiroyuki Takashi2), Kenji Marumo3), Minoru Yoshida2) |
| Affiliation |
1) Infection Control Team, Teikyo University Mizonokuchi Hospital
2) Infection Control Committee, Teikyo University Mizonokuchi Hospital
3) Division of Clinical Pathology, Infection Control Team, Showa University Fujigaoka Hospital |
| Publication |
J.J.C.M.: 22 (1), 60-65, 2012 |
| Received |
June 13, 2011 |
| Accepted |
December 5, 2011 |
| Abstract |
Stenotrophomonas maltophilia isolates from seven cases associated with respiratory tract infection or contamination was collected in our hospital for a short-term period of one month. In this study, we investigated whether S. maltophilia had been spread among inpatients at the hospital ward. S. maltophilia was isolated from the sputum samples of seven inpatients, while the organism was not at all isolated from other inpatient samples or water-borne-environments in the hospital ward. Based on molecular typing by random amplified polymorphic DNA (RAPD) technique using ERIC-2 primer and by pulsed-field gel electrophoresis, these isolates were identified as different strain types. Therefore, we denied the transmission among inpatients. S. maltophilia is often isolated from 65-aged or more inpatient samples during antimicrobial chemotherapy and its increased prevalence is likely to be associated with decreased usage of antiseptics for hand wash. Therefore, the pseudo-outbreak provided a reminder about active surveillance, antimicrobial pressure and hand hygiene. |
| Keywords |
Stenotrophomonas maltophilia, random amplified polymorphic DNA (RAPD) technique, pulsed-field gel electrophoresis (PFGE) method |
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