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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of Direct Latex Agglutination of Selective Broth for Detection of Group B Streptococcal Carriage in Pregnant Women |
| Language |
J |
| AuthorList |
Makiko Baden, Tomohiro Higashiyama, Toshiyuki Ikemoto, Yoshikatsu Okada |
| Affiliation |
Division of Microbiology, Central Laboratory, Osaka Medical College Hospital |
| Publication |
J.J.C.M.: 26 (4), 297-303, 2016 |
| Received |
March 28, 2016 |
| Accepted |
May 9, 2016 |
| Abstract |
The Centers for Disease Control and Prevention guidelines for the prevention of perinatal Group B Streptococcus (GBS) infections recommend that pregnant women should be tested at 35 to 37 weeks of gestation for carriage of GBS by enriched culture broth (Todd-Hewitt broth supplemented with selective antibiotics; LIM or TransVag broth), followed by subculture on an appropriate agar plate (LIM-BA). However, this process can be labor-intensive and typically requires 2 or 3 days to provide results, because the broth must be incubated for 18 to 24 hours and then subcultured on agar plates. The aim of our study is to evaluate LIM broth with direct GBS antigen detection (LIM-AG) as an alternative to LIM-BA for GBS screening in pregnant women. Although domestically there has been no study so far about this method, a number of studies conducted outside the country have shown this method to be sensitive, cost-effective, decreasing test turnaround time, and easier to perform in the laboratory. A total of 50 specimens were examined, with 4 positive for GBS. All four isolates were recovered on direct agar media for 24 hours, of which 3 were isolated both from LIM-AG and LIM-BA. One of the specimens was positive by direct culture method only, which revealed heavy growth Enterococcus faecalis. Results from our study suggest that LIM-AG would be an appropriate alternative to LIM-BA and also it is more efficient to use direct culture method concurrently with LIM-AG to avoid false-negative study caused by overgrowth of E. faecalis. |
| Keywords |
Enterococcus faecalis |
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