 |
The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Existence ratio of enterohemorrhagic E. coli, estimated using the ratio of the uidA to stx1/stx2 genes as an indicator |
| Language |
J |
| AuthorList |
Tomoko Kitahashi1), Junko Yoshihara2), Ryoko Shimamura1), Junko Ohki1) |
| Affiliation |
1) Chiba City Institute of Health and Environment
2) Chiba City Public Health Center |
| Publication |
J.J.C.M.: 26 (4), 304-310, 2016 |
| Received |
November 6, 2015 |
| Accepted |
June 2, 2016 |
| Abstract |
The existence ratios of enterohemorrhagic Escherichia coli (EHEC) to other Escherichia coli species were estimated in feces using the uidA gene encoding β-glucuronidase as an indicator. The uidA and stx1/stx2 genes existed at comparable ratios (differences in Ct values), as determined by real-time PCR using a mixture of equal amounts of EHEC reference and Escherichia coli strains. Thus, our method can be applied to isolate EHEC (O-antigens), for which selective media cannot be employed. |
| Keywords |
uidA, stx |
|
