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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of improved CHROMagar MRSA chromogenic media in the detection of thymidine-dependent small colony variants of methicillin- resistant Staphylococcus aureus |
| Language |
J |
| AuthorList |
Mitsuru Nishio, Yuki Miyaki, Yuriko Ogawa, Takato Osugi |
| Affiliation |
Department of Clinical Laboratory, Komaki City Hospital |
| Publication |
J.J.C.M.: 27 (4), 298-305, 2017 |
| Received |
May 22, 2017 |
| Accepted |
July 18, 2017 |
| Abstract |
Small colony variants (SCVs) constitute a slow-growing subpopulation of bacteria with distinctive phenotypic and pathogenic traits. Phenotypically, SCVs have a typical colony morphology and unusual biochemical characteristics. Therefore, it is difficult to detect and identify SCVs. A composition of the culture media of the CHROMagaer MRSA was improved so as to facilitate the growth of thymidine-dependent SCVs-MRSA. We compared the growth performance of the SCVs-MRSA3 strain in eight kinds of culture media. We found that MRSA could grow only on improved CHROMagaer MRSA (improved CHROM). The MRSA growth ability was assessed using the Miles & Misra method, and the results showed that the SCVs and normal MRSA grew to similar dilution ratio on improved CHROM. We also evaluated improved CHROM using 150 clinical specimens. After 24 h of incubation, the sensitivity, specificity, positive predictive values, and negative predictive values were all found to be 100%. The MRSA detection of improved CHROM was useful in clinical specimens. We conclude that SCVs-MRSA can be easily detected using improved CHROM. |
| Keywords |
Methicillin-resistant Staphylococcus aureus（MRSA） |
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