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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.27 No.4 contents] Japanese / English |
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| ArticleTitle | Two cases of non-tubeculosis mycobacteria misidentified as Mycobacterium avium complex with GENECUBE |
| Language | J |
| AuthorList | Yuka Yamashita1), Yuiko Morokuma1), Tomomi Mochimaru1), Ruriko Nishida1,2), Makiko Kiyosuke1), Taeko Hotta1), Masayuki Murata3), Nobuyuki Shimono4), Dongchon Kang1,5) |
| Affiliation |
1) Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital 2) Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Science 3) Department of General Internal Medicine, Kyushu University Hospital 4) Center for the Study of Global Infection, Kyushu University Hospital 5) Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences |
| Publication | J.J.C.M.: 27 (4), 306-312, 2017 |
| Received | December 9, 2016 |
| Accepted | April 26, 2017 |
| Abstract | We report two cases of non-tuberculosis mycobacteria misidentified as Mycobacterium avium complex (MAC) with GENECUBE (Toyobo), which is an instrument used for mycobacterium polymerase chain reaction (PCR). The first case is an 80s female. Acid-fast bacilli (AFB) were detected in a liquid medium of sputum culture after 23 days of incubation. A GENECUBE analysis of the isolate indicated MAC. However, the strain was slow-growing and formed yellow-pigmented non-tuberculosis mycobacteria colonies on a two percent Ogawa's medium. This strain was identified as Mycobacterium lentiflavum by 16SrRNA, dnaJ and hsp65 gene sequencing. The second case is a 30s male with human immunodeficiency virus infection. Ziehl Neelsen staining of bone marrow, sputum and stool were positive. AFB were detected in blood culture and a liquid medium of bone marrow. A GENECUBE analysis of the specimens and isolates indicated MAC, but in all samples, the cultures on solid media did not show any colony formation over the six months. These strains were identified as Mycobacterium genavense by 16SrRNA, dnaJ and hsp65 gene sequencing. This is the first report about misidentifications of MAC with GENECUBE in Japan. PCR reaction has a merit in its rapid reaction directly from samples and culture bottles, but also has a demerit not to confirm validity by colony observation. We should make final identification in a comprehensive manner by paying attention to clinical findings and other examination results. |
| Keywords | Mycobacterium lentiflavum, Mycobacterium genavense, GENECUBE |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |