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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.28 No.2 contents] Japanese / English |
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| ArticleTitle | Evaluation of accuracy and availability of DPS192ix for the antimicrobial susceptibility testing of gram-negative β-lactamase-producing Enterobacteriaceae |
| Language | J |
| AuthorList | Kenichiro Ohnuma1,2), Tatsuya Nakamura1,3), Mari Kusuki1,3), Nami Ishida1), Saori Kobayashi1), Mayu Fujiwara1), Masako Nishida1), Goh Ohji1,4), Issei Tokimatsu3), Nobuhide Hayashi5), Yuji Nakamachi1), Jun Saegusa1,2) |
| Affiliation |
1) Department of Clinical Laboratory, Kobe University Hospital, Japan 2) Division of Laboratory Medicine, Kobe University Graduate School of Medicine, Japan 3) Department of Infection Prevention and Control, Kobe University Hospital, Japan 4) Division of Infectious Diseases Therapeutics, Department of Microbiology and Infectious Diseases, Kobe University Graduate School of Medicine, Japan 5) Department of Medical Technology, Faculty of Health Sciences, Kobe Tokiwa University |
| Publication | J.J.C.M.: 28 (2), 112-118, 2018 |
| Received | June 12, 2017 |
| Accepted | December 6, 2017 |
| Abstract | The rapid detection of antimicrobial resistant strain, especially carbapenemase-producing Enterobacteriaceae (CPE), is important for the effective treatment of infectious diseases. In the present study, we evaluated availability of the automatic analyzer DPS192ix. The susceptibilities of 89 strains including 44 strains of CPE to 9 antibiotics were measured with DPS192ix using EP01 panel, and compared with DP panel as reference methods. The rates of agreement within±1 dilution ranged from 96.6% to 100%, and of category agreement ranged from 91.1% to 98.8%. It was noteworthy that less than 1.1% of strains showed very major error and major error. In addition, we could detect resistant strain to some antibiotics after only 3 hours of culture beginning by confirming reports from DPS192ix once every hour. Furthermore, MIC value over than 4 μg/ml of faropenem at 5 hours after culture beginning as a screening test of CPE had a sensitivity of 95.5% and a specificity of 87.7%. In conclusion, DPS192ix is useful to reduce turnaround time for reporting drug-resistant strain and to choose the appropriate drugs for the treatment of infectious diseases caused by beta-lactamase producing bacteria. |
| Keywords | faropenem |
| Copyright © 2002 The Japanese Society for Clinical Microbiology All rights reserved. |