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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Evaluation of the modified ESBL NDP test for a rapid detection of extended-spectrum β-lactamase (ESBL) produced by Enterobacteriaceae |
| Language |
J |
| AuthorList |
Kohei Uechi1,2), Isamu Nakasone3), Mikako Nonaka1), Momoko Arakaki1), Takaaki Tome1), Jiro Fujita2), Shiro Maeda1) |
| Affiliation |
1) Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital, Okinawa, Japan
2) Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan
3) Control and Prevention of Infectious Disease, University of the Ryukyus Hospital, Okinawa, Japan |
| Publication |
J.J.C.M.: 28 (3), 173-182, 2018 |
| Received |
June 30, 2017 |
| Accepted |
December 4, 2017 |
| Abstract |
Available conventional methods for clinical microbiology laboratories to detect Extended-Spectrum β-lactamases (ESBLs) producing Enterobacteriaceae isolates are time consuming because of the period required for bacterial culture. Additionally, the methods possibly fail to detect the production of ESBLs, especially when other β-lactamases, such as AmpC β-lactamases, are simultaneously produced. The ESBL NDP test is a rapid method for detection of ESBLs in Enterobacteriaceae. In this study, we developed a modified ESBL NDP test and evaluated it using a commercialy available CTX/CVA disk instead of tazobactam. The modified ESBL NDP test was applied to cultured clinical isolates from various specimens, 127 ESBLs producing and 25 non-producing Enterobacteriaceae isolates, that were resistant to at least one of third generation cephalosporin. The sensitivity and specificity of this test for the cultured isolates were 92.1% and 88.0%, respectively. Moreover, 81.7% (98/117isolates) of ESBLs positive isolates were detected within 30 minutes. We also evaluated the usefulness of the test using 102 positive blood culture samples for Gram-negative bacteria. Among the samples examined, 21 isolates were identified as CTX-M-type ESBLs producing Enterobacteriaceae, and were positive on the modified ESBL NDP test. The complete cycle of the modified ESBL NDP test finished within one hour. From these observations, we conclude that the modified ESBL NDP test is a useful and rapid detection method for ESBLs producing Enterobacteriaceae isolates from both bacterial colonies and positive blood culture samples. |
| Keywords |
Extended-Spectrum β-lactamases (ESBLs), modified ESBL NDP test |
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