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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Identification of filamentous fungi detected from respiratory specimens and molecular epidemiology of Aspergillus fumigatus |
| Language |
J |
| AuthorList |
Hisaki Shimosaka1), Yuta Hayashi2), Takashi Umeyama3), Atsushi Izumi1), Hitoshi Kinoshita1), Shigeki Nakamura3), Yasuko Nakayama3), Yurika Dantsuji3), Yoshitsugu Miyazaki3), Kenji Ogawa2) |
| Affiliation |
1) Department of Clinical Laboratory, National Hospital Organization Higashinagoya National Hospital
2) Department of Respiratory Medicine, National Hospital Organization Higashinagoya National Hospital
3) Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases |
| Publication |
J.J.C.M.: 28 (3), 183-191, 2018 |
| Received |
June 30, 2017 |
| Accepted |
December 25, 2017 |
| Abstract |
In our hospital, as there were many filamentous fungi detected from respiratory specimens, we had to investigate whether there was any contamination source in a particular place. In this study, 59 strains of filamentous fungi separated in the bacteria laboratory of our hospital during the 7 months from May 2015 to November 2015 were identified by phenotype analysis and nucleotide sequence analysis of the genes. In addition, Aspergillus fumigatus was genotyped using the MLST (multilocus sequence typing) method and investigated together with clinical information. The results of the identification of 59 strains were 33 strains (55.9%) of A. fumigatus, 10 strains (16.9%) of Aspergillus niger, 5 strains (8.5%) of Aspergillus tubingensis (cryptic species of A. niger), 2 strains of Aspergillus flavus(3.4%), Neosartorya hiratsukae (cryptic species of A. fumigatus), Emericella quadrilineata (cryptic species of Aspergillus nidulans) each one strain (1.7%), 7 filamentous fungi other than Aspergillus genus (11.9%). 15 types of ST (sequence type) of A. fumigatus were detected, including 10 known types and 5 newly found types. Many ST were detected in this MLST analysis, contamination from a specific place was considered negative, because there was no commonality in sampling time and place, clinical information among the same ST. The reason why many filamentous fungi are detected in our hospital is as follows. There are many targeted patients, clinical laboratory technicians devise cultivation, and clinicians have high awareness to filamentous fungus culture. |
| Keywords |
Aspergillus fumigatus, MLST, respiratory tract specimens, chronic pulmonary aspergillosis |
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