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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
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[Vol.28 No.3 contents] Japanese / English |
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| ArticleTitle | The Performance Evaluation of the Verigene System with a Verigene Gram-positive and Gram-negative Blood Culture Tests for the Molecular Determination of Bacteria and Their Antimicrobial Resistances in Positive Blood Culture Bottles |
| Language | J |
| AuthorList | Hiromichi Suzuki1), Yuji Yaguchi2), Atsuo Ueda3), Hiroshi Koganemaru4), Shigeyuki Notake3), Eri Morishita5), Etsuko Shaku6), Mitsuyo Takasu6), Keiichi Uemura7), Hideki Nakao5), Yusaku Akashi1), Yuta Yoshimoto2), Yoshibumi Akatsu8), Yuji Ito9), Naoto Ishimaru10), Daisuke Shichi11), Takuya Watanabe12), Kiyoko Tamai2), Katsunori Yanagihara13) |
| Affiliation |
1) Division of Infectious Diseases, Department of Medicine/ Department of Clinical Laboratory Medicine, Tsukuba Medical Center Hospital 2) Miroku Medical Laboratory Inc. 3) Department of Clinical Laboratory, Tsukuba Medical Center Hospital 4) Department of Infectious Diseases, University of Tsukuba Hospital 5) Department of Clinical Laboratory, Akashi Medical Center 6) Department of Clinical Laboratory, Seirei Hamamatsu General Hospital 7) Department of Clinical Laboratory, Chutoen General Medical Center 8) Department of Clinical Laboratory, Hitachi General Hospital 9) Department of General Internal Medicine, Chutoen General Medical Center 10) Department of General Internal Medicine, Akashi Medical Center 11) Department of Infectious Diseases and Rheumatology, Seirei Mikatahara General Hospital 12) Department of General Internal Medicine, Seirei Hamamatsu General Hospital 13) Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences |
| Publication | J.J.C.M.: 28 (3), 192-202, 2018 |
| Received | August 15, 2017 |
| Accepted | January 30, 2018 |
| Abstract | We prospectively evaluated the performance of the Verigene System with the Verigene Gram-positive Blood Culture Test (BC-GP) and Verigene Gram-negative Blood Culture Test (BC-GN) in positive blood culture bottles between July 2014 and March 2016. The concordance rates were evaluated with phenotypic identification methods and conventional molecular examinations. In total, we evaluated 272 positive blood cultures bottles (282 strains), and the median duration between the initiation of the blood culture incubation and the reporting of the Verigene system result was 22.7 h (interquartile range 19.1-29.2 h). For the evaluation with the BC-GP, the concordance rates were 99.0% (103/104) for bacterial identifications and 100% (53/53) for drug resistance. For the evaluation with the BC-GN, the concordance rate was 97.8% (174/178) for bacterial identifications, and antimicrobial resistance genes were identified in 15 bottles (blaCTX-M: 13, blaIMP: 2). For additional evaluations of the BC-GN, we collected 96 cefotaxime-non-susceptible strains (Escherichia coli: 82, Klebsiella pneumoniae: 7, Klebsiella oxytoca: 3, Peroteus spp.: 4) that had been isolated from patients with bacteremia at 6 facilities, and resistance genes were identified using the Verigene system with the BC-GN in 88 strains (91.7%). All of the resistance genes were confirmed with conventional molecular examinations. In our current study, we confirmed that Verigene system could promptly identify bacteria and resistance genes directly from positive blood culture bottles. |
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