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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Rapid identification of Clostridium tetani from drain fluid of a patient with strangulated ileus, using mass spectrophotometry and DNA sequencing |
| Language |
J |
| AuthorList |
Kyohei Kato1), Zenzo Nagasawa2), Taeko Narita1), Yumiko Funashima2), Kenichi Sato2), Tetsuhiro Harada1), Yusuke Kawashima3), Tsukuru Umemura1,2) |
| Affiliation |
1) Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital
2) Department of Medical Technology and Sciences, International University of Health and Welfare, Fukuoka, Japan
3) Department of Surgery, Medical Kouhoukai Takagi Hospital |
| Publication |
J.J.C.M.: 28 (3), 213-218, 2018 |
| Received |
October 23, 2017 |
| Accepted |
January 15, 2018 |
| Abstract |
An 80s woman who had abdominal distention and melena, was emergently referred to an academic medical center for the treatment of deteriorated symptoms. Computed tomography showed intestinal gas and intestinal fluid retention. Emergency operation was performed as ileus. Postoperative drain fluid was examined for bacterial culture. The fluid was anaerobically cultured in "KBM" Anaerobic Rabbit Blood Agar medium. A tiny amount of film-like colony appeared on day 2 of culture. Gram staining and spore staining of colonies revealed distinctive drumstick appearance. MALDI-TOF MS identified Clostridium tetani as the causative microorganism. The diagnosis of Tetanus was confirmed by 16S rRNA gene analysis and toxin-producing gene detected by PCR. In conclusion, MALDI-TOF MS and gene sequence analysis are new relevant technologies to make rapid, accurate diagnosis of Tetanus. |
| Keywords |
Clostridium tetani, Tetanus, Tetanospasmin, MALDI Biotyper |
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