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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Hemolysis inhibition of Enterococcus faecalis against Staphylococcus aureus |
| Language |
J |
| AuthorList |
Atsushi Kasai, Shiryu Takemura |
| Affiliation |
Department of Clinical Laboratory, National Hospital Organization Sendai Nishitaga Hospital |
| Publication |
J.J.C.M.: 28 (3), 219-224, 2018 |
| Received |
December 12, 2017 |
| Accepted |
March 13, 2018 |
| Abstract |
Clinical isolates of Enterococcus faecalis that were used as a negative control in a CAMP test inhibited β-hemolysis by Staphylococcus aureus ATCC 25923. S. aureus produces α-, β-, γ-, and δ-hemolysin. β-hemolysin does not cause β-hemolysis at 37°C and γ-hemolysin is inhibited by agar, so β-hemolysis by S. aureus ATCC 25923 is presumably caused by α- or δ-hemolysin. Based on the results of phenotypic discrimination, a strain of S. aureus producing α-hemolysin alone and a strain producing δ-hemolysin alone were collected, and a hemolysis inhibition test was conducted with 8 strains of E. faecalis that inhibit β-hemolysis by S. aureus ATCC 25923. Results indicated that all 8 strains inhibited hemolysis by δ-hemolysin. Some E. faecalis strains produce enterocin, which has antimicrobial action. A look at the enterocin activity of the 8 E. faecalis strains revealed that they had no antimicrobial action against the S. aureus strain producing δ-hemolysin alone. Therefore, this study has indicated that the mechanism by which E. faecalis inhibits hemolysis does not involve a quantitative decrease in δ-hemolysin due to antimicrobial action. |
| Keywords |
Staphylococcus aureus, Enterococcus faecalis, δ-hemolysin |
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