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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Investigation of New Direct Tube Coagulase Test using Centrifuged Supernatant of Blood Culture |
| Language |
J |
| AuthorList |
Makito Tanaka1), Masaaki Shinagawa1), Shinya Nirasawa1), Masachika Saeki1), Yuki Yakuwa1), Yuki Sato1), Satoshi Takahashi1,2) |
| Affiliation |
1) Division of Laboratory Medicine, Sapporo Medical University Hospital, Japan
2) Department of Infection Control and Laboratory Medicine, Sapporo MedicalUniversity School of Medicine, Japan |
| Publication |
J.J.C.M.: 29 (1), 7-11, 2018 |
| Received |
April 25, 2018 |
| Accepted |
August 10, 2018 |
| Abstract |
It is very important to identify Staphylococcus aureus rapidly from a bottle that has become positive for blood culture. In the Clinical Microbiology procedures handbook 4th, Direct Tube Coagulase Test (DTCT) have been recommended for testing the identification of S. aureus from a blood culture bottles. However, there are two problems in this method. One of that there is no definition about the test condition such as sample volume and reaction time. And the other, it is difficult to judge at an early point due to contamination of the blood cell component. Thus, we evaluated usefulness of the new DTCT using culture supernatant as a specimen. Sixty-three blood culture specimens that detected S. aureus were tested. As a result, it was shown that the best condition of new DTCT was to mix 500 μL of rabbit plasma with 300 μL of culture supernatant. The sensitivity of detection at 0.5, 1, 2, 4 and 24 h time points of the new DTCT using the best test condition was 78, 86, 94, 97 and 100%, respectively. On the other hand, the specificity of detection at 24 h time point was 100%. Furthermore, the sensitivity of new DTCT was no significant difference between aerobic and anaerobic culture. Similarly, there was no significant difference between methicillin-susceptible S. aureus and methicillin-resistant S. aureus. In conclusion, the new DTCT was useful as direct identification method of S. aureus from blood culture and suggested that contribute to prompt treatment of sepsis. |
| Keywords |
Staphylococcus aureus |
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