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The Journal of the Japanese Society for Clinical Microbiology |
Biblioraphy Information
| ArticleTitle |
Clinical evaluation of modified acridine orange staining solution for acid-fast bacilli smear microscopy and stained images of Mycobacterium type strains |
| Language |
J |
| AuthorList |
Yuriko Igarashi1), Kinuyo Chikamatsu1), Akio Aono1), Katsue Motohashi2), Hitomi Nakai2), Hideki Aoi2), Kazue Mizuno2), Hiroyuki Yamada1), Akiko Takaki1), Satoshi Mitarai1) |
| Affiliation |
1) Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association
2) Department of Clinical Microbiology, Fukujuji Hospital, Japan Anti-Tuberculosis Association |
| Publication |
J.J.C.M.: 29 (1), 12-16, 2018 |
| Received |
April 9, 2018 |
| Accepted |
October 19, 2018 |
| Abstract |
Modified Acristain (Kyokuto Pharmaceutical, Tokyo, Japan), acid-fast fluorescence staining solution using acridine orange, was modified. We evaluated the modified Acristain comparing with conventional staining methods. A total of 202 clinical samples were tested, including 187 and 15 sputa from tuberculosis and non-tuberculosis mycobacterium infection patients, respectively. Clinical samples were processed by conventional N-acetyl-L-cysteine and NaOH pretreatment method and re-suspended in phosphate buffer. Smear samples using these suspensions were examined with three staining methods; modified Acristain, conventional Acristain (Kyokuto Pharmaceutical) and Auramine O (Mutoh Chemicals) staining. The modified Acristain showed 93.6% of overall agreement between Acristain and Auramine O stain. Acristain showed 92.1% of overall agreement with Auramine O stain. Comparing the modified Acristain with the conventional Acristain, 35 samples showed higher smear positivity in the modified Acristain and 5 samples were vise versa.
In conclusion, the modified Acristain was considered useful as acid-fast fluorescence staining solution for smear microscopy with appropriate accuracy. The modified Acristain could reduce nonspecific glowing background and showed stronger fluorescence in several Mycobacterium type-strains compared with the conventional Acristain, and it showed high concordance among conventional acid-fast bacilli staining methods. |
| Keywords |
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